显然,这说明水提取物的一 oblongifolia leaves posses significant antimicrobial activity against gram positive, gram negative and fungal species. oblongifolia叶片具有的显着抗革兰氏阳性抗菌活性,革兰阴性和真菌。 Fraction AP2 is slightly active against B. AP2结构比例还积极对乙略 subtilis and A.枯草芽孢杆菌和A. fumigatus but fractions (AP3) was found highly active by producing clear zones of inhibition against B.但烟分数(AP3)被发现生产高度对二区的抑制活性明显 subtilis and A.枯草芽孢杆菌和A. niger as shown in Table 3.尼日尔如表三所示 Moreover, fraction AP4 was found moderately active against bacteria such as Kl.此外,分数为AP4发现适度对特列斯的明细表Kl活性细菌。 pneumoniae, S.肺炎,第 aureus, B.金黄色葡萄球菌,乙 subtilis and fungi including A.枯草芽孢杆菌和真菌包括A niger, A.尼日尔,答 fumigatus and A.烟和A. fla6us. fla6us。 Fraction AP1 did not show any activity against bacteria and fungi.的确显示分数为AP1的活动不是针对任何细菌和真菌。 E.大肠杆菌 coli, Kl.大肠杆菌,吉隆坡。 aerogenes and P.产气和周五 expansum were found ineffective against aqueous extract as well as all fractions of I. expansum的被发现无效水提取物对,以及我所有的分数 oblongifolia leaves. oblongifolia叶。 The antimicrobial effect, expressed as MIC in the serial dilution broth method, most of the organisms tested were not inhibited by concentration of up 100 mg/ml with fraction AP1 and AP2.该抗菌效果,快速的肉汤稀释法的MIC在串行,现在身体不是由被organismes高达100毫克/毫升浓度抑制率与AP1和AP2结构。 The inhibition of micro-organism growth is most pronounced for B.该微生物用于抑制微生物的生长,是现在宣布乙 subtilis and A.枯草芽孢杆菌和A. niger with Fraction AP3 of I.尼日尔与AP3分数是我 oblongifolia leaves. oblongifolia叶。 However, fraction AP4 exhibit a weak antibacterial but moderate antifungal activity.可是,该AP4图表分数是一个软弱的抗菌而温和的抗真菌活性。 This observation provide strong circumstantial evidence that antifungal small proteins/peptides play an important role in a plants antimicrobial defence system.这一观察提供有效的间接证据表明,小强抗真菌蛋白/肽,发挥抗菌植物防御系统的一个重要的角色。To our knowledge, this study is the first report on purification and characterization of antimicrobial small protein/peptide from I.就我们所知,这项研究是关于抗菌净化和从I /小肽蛋白定性的第一次报告 oblongifolia leaves in this country. oblongifolia离开这个国家。 This study provides considerable scope in exploiting the local indigenous resources for isolation of antibacterial/antifungal peptides or smaller proteins.本研究利用提供了隔离的当地土著的资源相当大的范围抗菌/防霉较小的肽或蛋白质。 Further work is under progress in this Laboratory for the determination of amino acid composition of active small protein/peptide, and results will be reported in near future.进一步的工作是根据在此氨基酸组成的小蛋白活性测定实验室的进展/肽,并将在不久的将来,报告的结果。